TY  - JOUR
AU  - Savic, Jasna
AU  - Pavlović, Jelena
AU  - Stanojević, Miloš
AU  - Bosnić, Predrag
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1911
AB  - Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.
PB  - MDPI
T2  - Plants
T1  - Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves
SP  - 1660
VL  - 12
DO  - 10.3390/ plants12081660
ER  - 

@article{
author = "Savic, Jasna and Pavlović, Jelena and Stanojević, Miloš and Bosnić, Predrag and Kostić Kravljanac, Ljiljana and Nikolić, Nina and Nikolic, Miroslav",
year = "2023",
abstract = "Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.",
publisher = "MDPI",
journal = "Plants",
title = "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves",
pages = "1660",
volume = "12",
doi = "10.3390/ plants12081660"
}

Savic, J., Pavlović, J., Stanojević, M., Bosnić, P., Kostić Kravljanac, L., Nikolić, N.,& Nikolic, M.. (2023). Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants
MDPI., 12, 1660.
https://doi.org/10.3390/ plants12081660

Savic J, Pavlović J, Stanojević M, Bosnić P, Kostić Kravljanac L, Nikolić N, Nikolic M. Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants. 2023;12:1660.
doi:10.3390/ plants12081660 .

Savic, Jasna, Pavlović, Jelena, Stanojević, Miloš, Bosnić, Predrag, Kostić Kravljanac, Ljiljana, Nikolić, Nina, Nikolic, Miroslav, "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves" in Plants, 12 (2023):1660,
https://doi.org/10.3390/ plants12081660 . .

TY  - CONF
AU  - Trailović, Maja
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - TODIC, Slavica
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2140
AB  - Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2140
ER  - 

@conference{
author = "Trailović, Maja and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Pavlović, Jelena and Bosnić, Predrag and TODIC, Slavica and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2140"
}

Trailović, M., Kostić Kravljanac, L., Stanojević, M., Pavlović, J., Bosnić, P., TODIC, S.,& Nikolic, M.. (2022). Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2140

Trailović M, Kostić Kravljanac L, Stanojević M, Pavlović J, Bosnić P, TODIC S, Nikolic M. Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

Trailović, Maja, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Pavlović, Jelena, Bosnić, Predrag, TODIC, Slavica, Nikolic, Miroslav, "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

TY  - CONF
AU  - Carballo Méndez, Fernand
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2120
AB  - Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Duration of priming with silicon modulates antioxidative response of wheat to salinity stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2120
ER  - 

@conference{
author = "Carballo Méndez, Fernand and Bosnić, Predrag and Bosnic, Dragana and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Nikolic, Miroslav",
year = "2022",
abstract = "Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2120"
}

Carballo Méndez, F., Bosnić, P., Bosnic, D., Nikolić, N., Kostić Kravljanac, L., Stanojević, M.,& Nikolic, M.. (2022). Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2120

Carballo Méndez F, Bosnić P, Bosnic D, Nikolić N, Kostić Kravljanac L, Stanojević M, Nikolic M. Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

Carballo Méndez, Fernand, Bosnić, Predrag, Bosnic, Dragana, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Nikolic, Miroslav, "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

TY  - CONF
AU  - Kostić, Igor
AU  - Milenkovic, Ivan
AU  - Nikolić, Nina
AU  - Milanovic, Slobodan
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Paravinja, Ana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2112
AB  - Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.
PB  - ISSAG and AgCenterLSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2112
ER  - 

@conference{
author = "Kostić, Igor and Milenkovic, Ivan and Nikolić, Nina and Milanovic, Slobodan and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Paravinja, Ana",
year = "2022",
abstract = "Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.",
publisher = "ISSAG and AgCenterLSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2112"
}

Kostić, I., Milenkovic, I., Nikolić, N., Milanovic, S., Kostić Kravljanac, L., Bosnić, P.,& Paravinja, A.. (2022). Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenterLSU., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112

Kostić I, Milenkovic I, Nikolić N, Milanovic S, Kostić Kravljanac L, Bosnić P, Paravinja A. Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

Kostić, Igor, Milenkovic, Ivan, Nikolić, Nina, Milanovic, Slobodan, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Paravinja, Ana, "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Kirkby, Ernest A.
AU  - Nikolic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1425
AB  - Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Interactions of Silicon With Essential and Beneficial Elements in Plants
VL  - 12
DO  - 10.3389/fpls.2021.697592
ER  - 

@article{
author = "Pavlović, Jelena and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Kirkby, Ernest A. and Nikolic, Miroslav",
year = "2021",
abstract = "Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Interactions of Silicon With Essential and Beneficial Elements in Plants",
volume = "12",
doi = "10.3389/fpls.2021.697592"
}

Pavlović, J., Kostić Kravljanac, L., Bosnić, P., Kirkby, E. A.,& Nikolic, M.. (2021). Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fpls.2021.697592

Pavlović J, Kostić Kravljanac L, Bosnić P, Kirkby EA, Nikolic M. Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science. 2021;12.
doi:10.3389/fpls.2021.697592 .

Pavlović, Jelena, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Kirkby, Ernest A., Nikolic, Miroslav, "Interactions of Silicon With Essential and Beneficial Elements in Plants" in Frontiers in Plant Science, 12 (2021),
https://doi.org/10.3389/fpls.2021.697592 . .

TY  - JOUR
AU  - Hajiboland, Roghieh
AU  - Sadeghzadeh, Noushin
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Tolra, Roser
AU  - Poschenrieder, Charlotte
AU  - Nikolic, Miroslav
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1351
AB  - Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Selenium activates components of iron acquisition machinery in oilseed rape roots
EP  - 586
IS  - 1-2
SP  - 569
VL  - 452
DO  - 10.1007/s11104-020-04599-w
ER  - 

@article{
author = "Hajiboland, Roghieh and Sadeghzadeh, Noushin and Bosnic, Dragana and Bosnić, Predrag and Tolra, Roser and Poschenrieder, Charlotte and Nikolic, Miroslav",
year = "2020",
abstract = "Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Selenium activates components of iron acquisition machinery in oilseed rape roots",
pages = "586-569",
number = "1-2",
volume = "452",
doi = "10.1007/s11104-020-04599-w"
}

Hajiboland, R., Sadeghzadeh, N., Bosnic, D., Bosnić, P., Tolra, R., Poschenrieder, C.,& Nikolic, M.. (2020). Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil
Springer, Dordrecht., 452(1-2), 569-586.
https://doi.org/10.1007/s11104-020-04599-w

Hajiboland R, Sadeghzadeh N, Bosnic D, Bosnić P, Tolra R, Poschenrieder C, Nikolic M. Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil. 2020;452(1-2):569-586.
doi:10.1007/s11104-020-04599-w .

Hajiboland, Roghieh, Sadeghzadeh, Noushin, Bosnic, Dragana, Bosnić, Predrag, Tolra, Roser, Poschenrieder, Charlotte, Nikolic, Miroslav, "Selenium activates components of iron acquisition machinery in oilseed rape roots" in Plant and Soil, 452, no. 1-2 (2020):569-586,
https://doi.org/10.1007/s11104-020-04599-w . .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Pavlicević, Milica
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1281
AB  - Both calcium (Ca2+) and silicon (Si) improve plant performance under salt (NaCl) stress. Although these two mineral elements share numerous similarities, the information on how their extracellular interactions in the root apoplast affect uptake of sodium (Na+) is still lacking. Here, we investigated the effect of high Si supply in the bioavailable form of monosilicic acid (H4SiO4) on the activity of Ca2+ in the external root solution, and subsequent root uptake and compartmentation of Na in maize (Zea mays L.). In the short-term experiments (6 h), 14-d-old maize plants were exposed to various concentrations of Ca2+ at three different pH-values (6.5, 7.5, and 8.5) and two Si concentrations, i.e., low (1 mM) and high (4 mM) supply of H4SiO4. The activity of Ca2+ and Na+ in the external solution as well as the root concentrations of total and cell sap and BaCl2-exchangeble apoplastic fractions of both elements were analyzed. The pH of the nutrient solution affected neither the ion activities nor the root accumulation of both Ca2+ and Na+. At higher pH values (7.5 and 8.5) the interactions of Ca2+ and Si at high Si supply led to a decrease of Ca2+ activity and, hence, an increase of Na+ : Ca2+ activity ratio in the external root solution. Concomitantly, despite the elevated exchangeable apoplastic fraction of both Ca2+ and Na+, the total and cell sap concentrations were remarkably decreased for Ca2+ and increased for Na+ by the addition of 4 mM H4SiO4. This work demonstrates that at high Si supply extracellular Ca-Si interactions leading to lowered activity of Ca2+ might rapidly compromise the ameliorative effect of Ca2+ on Na+ accumulation in roots. Practically, Si over-fertilization of saline and, in particular, sodic soils may further promote the accumulation of Na+ in root tissues hours after Si application and, hence, increase a potential risk of Na+ toxicity.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Plant Nutrition and Soil Science
T1  - High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity
EP  - 216
IS  - 2
SP  - 210
VL  - 182
DO  - 10.1002/jpln.201800153
ER  - 

@article{
author = "Bosnić, Predrag and Pavlicević, Milica and Nikolić, Nina and Nikolic, Miroslav",
year = "2019",
abstract = "Both calcium (Ca2+) and silicon (Si) improve plant performance under salt (NaCl) stress. Although these two mineral elements share numerous similarities, the information on how their extracellular interactions in the root apoplast affect uptake of sodium (Na+) is still lacking. Here, we investigated the effect of high Si supply in the bioavailable form of monosilicic acid (H4SiO4) on the activity of Ca2+ in the external root solution, and subsequent root uptake and compartmentation of Na in maize (Zea mays L.). In the short-term experiments (6 h), 14-d-old maize plants were exposed to various concentrations of Ca2+ at three different pH-values (6.5, 7.5, and 8.5) and two Si concentrations, i.e., low (1 mM) and high (4 mM) supply of H4SiO4. The activity of Ca2+ and Na+ in the external solution as well as the root concentrations of total and cell sap and BaCl2-exchangeble apoplastic fractions of both elements were analyzed. The pH of the nutrient solution affected neither the ion activities nor the root accumulation of both Ca2+ and Na+. At higher pH values (7.5 and 8.5) the interactions of Ca2+ and Si at high Si supply led to a decrease of Ca2+ activity and, hence, an increase of Na+ : Ca2+ activity ratio in the external root solution. Concomitantly, despite the elevated exchangeable apoplastic fraction of both Ca2+ and Na+, the total and cell sap concentrations were remarkably decreased for Ca2+ and increased for Na+ by the addition of 4 mM H4SiO4. This work demonstrates that at high Si supply extracellular Ca-Si interactions leading to lowered activity of Ca2+ might rapidly compromise the ameliorative effect of Ca2+ on Na+ accumulation in roots. Practically, Si over-fertilization of saline and, in particular, sodic soils may further promote the accumulation of Na+ in root tissues hours after Si application and, hence, increase a potential risk of Na+ toxicity.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Plant Nutrition and Soil Science",
title = "High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity",
pages = "216-210",
number = "2",
volume = "182",
doi = "10.1002/jpln.201800153"
}

Bosnić, P., Pavlicević, M., Nikolić, N.,& Nikolic, M.. (2019). High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity. in Journal of Plant Nutrition and Soil Science
Wiley-V C H Verlag Gmbh, Weinheim., 182(2), 210-216.
https://doi.org/10.1002/jpln.201800153

Bosnić P, Pavlicević M, Nikolić N, Nikolic M. High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity. in Journal of Plant Nutrition and Soil Science. 2019;182(2):210-216.
doi:10.1002/jpln.201800153 .

Bosnić, Predrag, Pavlicević, Milica, Nikolić, Nina, Nikolic, Miroslav, "High monosilicic acid supply rapidly increases Na accumulation in maize roots by decreasing external Ca2+ activity" in Journal of Plant Nutrition and Soil Science, 182, no. 2 (2019):210-216,
https://doi.org/10.1002/jpln.201800153 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Nikolic, Dragana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1220
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnic, Dragana and Bosnić, Predrag and Nikolic, Dragana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnic, D., Bosnić, P., Nikolic, D., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnic D, Bosnić P, Nikolic D, Nikolic M, Samardžić JT. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnic, Dragana, Bosnić, Predrag, Nikolic, Dragana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Kolasinac, Stefan M.
AU  - Bosnić, Predrag
AU  - Lekic, Slavoljub S.
AU  - Golijan, Jelena
AU  - Petrović, Tanja
AU  - Todorović, Goran
AU  - Kostic, Aleksandar Z.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1107
AB  - The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment
IS  - 11
VL  - 190
DO  - 10.1007/s10661-018-7047-y
ER  - 

@article{
author = "Kolasinac, Stefan M. and Bosnić, Predrag and Lekic, Slavoljub S. and Golijan, Jelena and Petrović, Tanja and Todorović, Goran and Kostic, Aleksandar Z.",
year = "2018",
abstract = "The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment",
number = "11",
volume = "190",
doi = "10.1007/s10661-018-7047-y"
}

Kolasinac, S. M., Bosnić, P., Lekic, S. S., Golijan, J., Petrović, T., Todorović, G.,& Kostic, A. Z.. (2018). Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(11).
https://doi.org/10.1007/s10661-018-7047-y

Kolasinac SM, Bosnić P, Lekic SS, Golijan J, Petrović T, Todorović G, Kostic AZ. Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment. 2018;190(11).
doi:10.1007/s10661-018-7047-y .

Kolasinac, Stefan M., Bosnić, Predrag, Lekic, Slavoljub S., Golijan, Jelena, Petrović, Tanja, Todorović, Goran, Kostic, Aleksandar Z., "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment" in Environmental Monitoring and Assessment, 190, no. 11 (2018),
https://doi.org/10.1007/s10661-018-7047-y . .

TY  - CONF
AU  - Djordjevic, Pavle
AU  - Kostić, Ljiljana
AU  - Bosnić, Predrag
AU  - Maksimović, Vuk
AU  - TODIC, Slavica
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2511
AB  - Low phosphorus (P) availability is a common constraint for crop productivity in both acid and
calcareous soils due to formation of insoluble P complexes. As far as we are aware, grapevine is not
considered a P-efficient species, whereas P deficiency is rarely reported under the field conditions.
We performed a field experiment under extremely low P conditions (Olsen P < 2 mg kg-1) using
own-designed rhizotron (80 cm depth), installed in 8-y-old vineyard with cultivar Chardonnay,
grafted on 5BB rootstock. The device enables easy access to the new intact roots while preventing
root drying. We collected the exudates from root tips every 2 h (during daytime) at the different
growth stages (i.e. shoots 10 cm, flowering, berries pea-size and veraison). At the same time, both
rhizosphere and bulk soil samples were collected and subjected to the sequential P analyses. The
results of HPLC analyses of the root exudates showed that citrate can be considered as the main
P-mobilizing compound, with clear seasonal and diurnal patterns: the highest anion exudation
rate was recorded at flowering (lower pick) and veraison (higher pick), and at each growth phase
in the morning (10 a.m.) and in the afternoon (4 p.m.), with a rapid depression at 1-3 p.m. Consequently,
the relative share of soluble P fractions (H2O- and NaHCO3-extractable) in the rhizosphere
was very high (20% of the total P fractions), leading to the leaf P concentration of 0.2% DW which
is above the critical P-deficient level (0.15% DW) for grapevine leaves at flowering.
PB  - Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade
C3  - Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade
T1  - Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2511
ER  - 

@conference{
author = "Djordjevic, Pavle and Kostić, Ljiljana and Bosnić, Predrag and Maksimović, Vuk and TODIC, Slavica and Nikolic, Miroslav",
year = "2018",
abstract = "Low phosphorus (P) availability is a common constraint for crop productivity in both acid and
calcareous soils due to formation of insoluble P complexes. As far as we are aware, grapevine is not
considered a P-efficient species, whereas P deficiency is rarely reported under the field conditions.
We performed a field experiment under extremely low P conditions (Olsen P < 2 mg kg-1) using
own-designed rhizotron (80 cm depth), installed in 8-y-old vineyard with cultivar Chardonnay,
grafted on 5BB rootstock. The device enables easy access to the new intact roots while preventing
root drying. We collected the exudates from root tips every 2 h (during daytime) at the different
growth stages (i.e. shoots 10 cm, flowering, berries pea-size and veraison). At the same time, both
rhizosphere and bulk soil samples were collected and subjected to the sequential P analyses. The
results of HPLC analyses of the root exudates showed that citrate can be considered as the main
P-mobilizing compound, with clear seasonal and diurnal patterns: the highest anion exudation
rate was recorded at flowering (lower pick) and veraison (higher pick), and at each growth phase
in the morning (10 a.m.) and in the afternoon (4 p.m.), with a rapid depression at 1-3 p.m. Consequently,
the relative share of soluble P fractions (H2O- and NaHCO3-extractable) in the rhizosphere
was very high (20% of the total P fractions), leading to the leaf P concentration of 0.2% DW which
is above the critical P-deficient level (0.15% DW) for grapevine leaves at flowering.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade",
journal = "Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade",
title = "Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2511"
}

Djordjevic, P., Kostić, L., Bosnić, P., Maksimović, V., TODIC, S.,& Nikolic, M.. (2018). Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment. in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade
Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2511

Djordjevic P, Kostić L, Bosnić P, Maksimović V, TODIC S, Nikolic M. Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment. in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2511 .

Djordjevic, Pavle, Kostić, Ljiljana, Bosnić, Predrag, Maksimović, Vuk, TODIC, Slavica, Nikolic, Miroslav, "Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment" in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2511 .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Jasnic, Jovana
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1106
AB  - Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Silicon mediates sodium transport and partitioning in maize under moderate salt stress
EP  - 687
SP  - 681
VL  - 155
DO  - 10.1016/j.envexpbot.2018.08.018
ER  - 

@article{
author = "Bosnić, Predrag and Bosnic, Dragana and Jasnic, Jovana and Nikolic, Miroslav",
year = "2018",
abstract = "Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Silicon mediates sodium transport and partitioning in maize under moderate salt stress",
pages = "687-681",
volume = "155",
doi = "10.1016/j.envexpbot.2018.08.018"
}

Bosnić, P., Bosnic, D., Jasnic, J.,& Nikolic, M.. (2018). Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 155, 681-687.
https://doi.org/10.1016/j.envexpbot.2018.08.018

Bosnić P, Bosnic D, Jasnic J, Nikolic M. Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany. 2018;155:681-687.
doi:10.1016/j.envexpbot.2018.08.018 .

Bosnić, Predrag, Bosnic, Dragana, Jasnic, Jovana, Nikolic, Miroslav, "Silicon mediates sodium transport and partitioning in maize under moderate salt stress" in Environmental and Experimental Botany, 155 (2018):681-687,
https://doi.org/10.1016/j.envexpbot.2018.08.018 . .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2149
AB  - Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.
PB  - University of Copenhagen, Denmark
C3  - XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
T1  - Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2149
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.",
publisher = "University of Copenhagen, Denmark",
journal = "XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark",
title = "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2149"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2149

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress" in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2141
AB  - The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.
PB  - University of Agricultural Sciences, GKVK, Bengaluru
C3  - 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
T1  - Silicon influence on plant ionome and mineral element transporters
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2141
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.",
publisher = "University of Agricultural Sciences, GKVK, Bengaluru",
journal = "7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India",
title = "Silicon influence on plant ionome and mineral element transporters",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2141"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
University of Agricultural Sciences, GKVK, Bengaluru..
https://hdl.handle.net/21.15107/rcub_rimsi_2141

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon influence on plant ionome and mineral element transporters" in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

TY  - JOUR
AU  - Nikolic, Miroslav
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - Stević, Nenad
AU  - Savić, Jasna
AU  - Hristov, Nikola
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/949
AB  - The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition
EP  - 148
SP  - 141
VL  - 553
DO  - 10.1016/j.scitotenv.2016.02.102
ER  - 

@article{
author = "Nikolic, Miroslav and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag and Stević, Nenad and Savić, Jasna and Hristov, Nikola",
year = "2016",
abstract = "The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition",
pages = "148-141",
volume = "553",
doi = "10.1016/j.scitotenv.2016.02.102"
}

Nikolic, M., Nikolić, N., Kostić Kravljanac, L., Pavlović, J., Bosnić, P., Stević, N., Savić, J.,& Hristov, N.. (2016). The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment
Elsevier, Amsterdam., 553, 141-148.
https://doi.org/10.1016/j.scitotenv.2016.02.102

Nikolic M, Nikolić N, Kostić Kravljanac L, Pavlović J, Bosnić P, Stević N, Savić J, Hristov N. The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment. 2016;553:141-148.
doi:10.1016/j.scitotenv.2016.02.102 .

Nikolic, Miroslav, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, Stević, Nenad, Savić, Jasna, Hristov, Nikola, "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition" in Science of the Total Environment, 553 (2016):141-148,
https://doi.org/10.1016/j.scitotenv.2016.02.102 . .

TY  - JOUR
AU  - Dinić, Zoran
AU  - Ugrinović, Milan
AU  - Bosnić, Predrag
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Miladinović, Miroslav
AU  - Jošić, Dragana
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2711
AB  - Phosphorus (P) is one of the major essential macronutrients for plant growth. Phosphate
solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and
inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study, endophytic bacteria were isolated from nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4 Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml -1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules
IS  - 51
DO  - 10.5937/ratpov51-6222
ER  - 

@article{
author = "Dinić, Zoran and Ugrinović, Milan and Bosnić, Predrag and Mijatović, Mirjana and Zdravković, Jasmina and Miladinović, Miroslav and Jošić, Dragana",
year = "2014",
abstract = "Phosphorus (P) is one of the major essential macronutrients for plant growth. Phosphate
solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and
inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study, endophytic bacteria were isolated from nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4 Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml -1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules",
number = "51",
doi = "10.5937/ratpov51-6222"
}

Dinić, Z., Ugrinović, M., Bosnić, P., Mijatović, M., Zdravković, J., Miladinović, M.,& Jošić, D.. (2014). Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad.(51).
https://doi.org/10.5937/ratpov51-6222

Dinić Z, Ugrinović M, Bosnić P, Mijatović M, Zdravković J, Miladinović M, Jošić D. Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo. 2014;(51).
doi:10.5937/ratpov51-6222 .

Dinić, Zoran, Ugrinović, Milan, Bosnić, Predrag, Mijatović, Mirjana, Zdravković, Jasmina, Miladinović, Miroslav, Jošić, Dragana, "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules" in Ratarstvo i povrtarstvo, no. 51 (2014),
https://doi.org/10.5937/ratpov51-6222 . .

TY  - CONF
AU  - Bosnić, Predrag
AU  - Savic, Jasna
AU  - Kostić, Ljiljana
AU  - Stevic, Nenad
AU  - Pavlović, Jelena
AU  - Lazic, Marina
AU  - Marjanovic Jeromela, Ana
AU  - Hristov, Nikola
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2504
AB  - Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2504
ER  - 

@conference{
author = "Bosnić, Predrag and Savic, Jasna and Kostić, Ljiljana and Stevic, Nenad and Pavlović, Jelena and Lazic, Marina and Marjanovic Jeromela, Ana and Hristov, Nikola and Nikolić, Nina and Nikolic, Miroslav",
year = "2013",
abstract = "Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2504"
}

Bosnić, P., Savic, J., Kostić, L., Stevic, N., Pavlović, J., Lazic, M., Marjanovic Jeromela, A., Hristov, N., Nikolić, N.,& Nikolic, M.. (2013). Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2504

Bosnić P, Savic J, Kostić L, Stevic N, Pavlović J, Lazic M, Marjanovic Jeromela A, Hristov N, Nikolić N, Nikolic M. Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

Bosnić, Predrag, Savic, Jasna, Kostić, Ljiljana, Stevic, Nenad, Pavlović, Jelena, Lazic, Marina, Marjanovic Jeromela, Ana, Hristov, Nikola, Nikolić, Nina, Nikolic, Miroslav, "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

TY  - JOUR
AU  - Postic, Dobrivoj
AU  - Starović, Mira
AU  - Popović, Tatjana
AU  - Bosnić, Predrag
AU  - Stanojkovic-Sebic, Aleksandra
AU  - Pivić, Radmila
AU  - Josic, Dragana
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/725
AB  - Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 mu gmL(-1) after 24h of cultivation and 4.08 to 26.4 mu gmL(-1) after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20 degrees C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Selection and rapd analysis of pseudomonas ssp. Isolates able to improve biological viability of potato seed tubers
EP  - 249
IS  - 1
SP  - 237
VL  - 45
DO  - 10.2298/GENSR1301237P
ER  - 

@article{
author = "Postic, Dobrivoj and Starović, Mira and Popović, Tatjana and Bosnić, Predrag and Stanojkovic-Sebic, Aleksandra and Pivić, Radmila and Josic, Dragana",
year = "2013",
abstract = "Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 mu gmL(-1) after 24h of cultivation and 4.08 to 26.4 mu gmL(-1) after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20 degrees C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Selection and rapd analysis of pseudomonas ssp. Isolates able to improve biological viability of potato seed tubers",
pages = "249-237",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301237P"
}

Postic, D., Starović, M., Popović, T., Bosnić, P., Stanojkovic-Sebic, A., Pivić, R.,& Josic, D.. (2013). Selection and rapd analysis of pseudomonas ssp. Isolates able to improve biological viability of potato seed tubers. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 237-249.
https://doi.org/10.2298/GENSR1301237P

Postic D, Starović M, Popović T, Bosnić P, Stanojkovic-Sebic A, Pivić R, Josic D. Selection and rapd analysis of pseudomonas ssp. Isolates able to improve biological viability of potato seed tubers. in Genetika-Belgrade. 2013;45(1):237-249.
doi:10.2298/GENSR1301237P .

Postic, Dobrivoj, Starović, Mira, Popović, Tatjana, Bosnić, Predrag, Stanojkovic-Sebic, Aleksandra, Pivić, Radmila, Josic, Dragana, "Selection and rapd analysis of pseudomonas ssp. Isolates able to improve biological viability of potato seed tubers" in Genetika-Belgrade, 45, no. 1 (2013):237-249,
https://doi.org/10.2298/GENSR1301237P . .

TY  - CONF
AU  - Poštić, Dobrivoj
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Popović, Tatjana
AU  - Bosnić, Predrag
AU  - Delić, Dušica
AU  - Jošić, Dragana
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2758
PB  - Beograd: Udruzenje mikrobioioga Srbije
C3  - Proceedings: 7th Balkan congress of microbiology and 8th congress of Serbian microbiologists, Microbiologia Balkanica 2011, Belgrade, October 25-29, 2011.
T1  - Sprouting duration and Pseudomonas spp. impact on biological viability of potato seed tubers
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2758
ER  - 

@conference{
author = "Poštić, Dobrivoj and Aleksić, Goran and Starović, Mira and Popović, Tatjana and Bosnić, Predrag and Delić, Dušica and Jošić, Dragana",
year = "2011",
publisher = "Beograd: Udruzenje mikrobioioga Srbije",
journal = "Proceedings: 7th Balkan congress of microbiology and 8th congress of Serbian microbiologists, Microbiologia Balkanica 2011, Belgrade, October 25-29, 2011.",
title = "Sprouting duration and Pseudomonas spp. impact on biological viability of potato seed tubers",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2758"
}

Poštić, D., Aleksić, G., Starović, M., Popović, T., Bosnić, P., Delić, D.,& Jošić, D.. (2011). Sprouting duration and Pseudomonas spp. impact on biological viability of potato seed tubers. in Proceedings: 7th Balkan congress of microbiology and 8th congress of Serbian microbiologists, Microbiologia Balkanica 2011, Belgrade, October 25-29, 2011.
Beograd: Udruzenje mikrobioioga Srbije..
https://hdl.handle.net/21.15107/rcub_rimsi_2758

Poštić D, Aleksić G, Starović M, Popović T, Bosnić P, Delić D, Jošić D. Sprouting duration and Pseudomonas spp. impact on biological viability of potato seed tubers. in Proceedings: 7th Balkan congress of microbiology and 8th congress of Serbian microbiologists, Microbiologia Balkanica 2011, Belgrade, October 25-29, 2011.. 2011;.
https://hdl.handle.net/21.15107/rcub_rimsi_2758 .

Poštić, Dobrivoj, Aleksić, Goran, Starović, Mira, Popović, Tatjana, Bosnić, Predrag, Delić, Dušica, Jošić, Dragana, "Sprouting duration and Pseudomonas spp. impact on biological viability of potato seed tubers" in Proceedings: 7th Balkan congress of microbiology and 8th congress of Serbian microbiologists, Microbiologia Balkanica 2011, Belgrade, October 25-29, 2011. (2011),
https://hdl.handle.net/21.15107/rcub_rimsi_2758 .

TY  - CONF
AU  - Bosnić, Predrag
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2765
C3  - Proceedings of the 7th Balkan Congress of Microbiology - Microbiologia Balkanica
T1  - Selection of Rhizospheric and Endophytic Phosphosolubilizing Bacteria
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2765
ER  - 

@conference{
author = "Bosnić, Predrag",
year = "2011",
journal = "Proceedings of the 7th Balkan Congress of Microbiology - Microbiologia Balkanica",
title = "Selection of Rhizospheric and Endophytic Phosphosolubilizing Bacteria",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2765"
}

Bosnić, P.. (2011). Selection of Rhizospheric and Endophytic Phosphosolubilizing Bacteria. in Proceedings of the 7th Balkan Congress of Microbiology - Microbiologia Balkanica.
https://hdl.handle.net/21.15107/rcub_rimsi_2765

Bosnić P. Selection of Rhizospheric and Endophytic Phosphosolubilizing Bacteria. in Proceedings of the 7th Balkan Congress of Microbiology - Microbiologia Balkanica. 2011;.
https://hdl.handle.net/21.15107/rcub_rimsi_2765 .

Bosnić, Predrag, "Selection of Rhizospheric and Endophytic Phosphosolubilizing Bacteria" in Proceedings of the 7th Balkan Congress of Microbiology - Microbiologia Balkanica (2011),
https://hdl.handle.net/21.15107/rcub_rimsi_2765 .