TY  - CONF
AU  - Carballo Méndez, Fernand
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2120
AB  - Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Duration of priming with silicon modulates antioxidative response of wheat to salinity stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2120
ER  - 

@conference{
author = "Carballo Méndez, Fernand and Bosnić, Predrag and Bosnic, Dragana and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Nikolic, Miroslav",
year = "2022",
abstract = "Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2120"
}

Carballo Méndez, F., Bosnić, P., Bosnic, D., Nikolić, N., Kostić Kravljanac, L., Stanojević, M.,& Nikolic, M.. (2022). Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2120

Carballo Méndez F, Bosnić P, Bosnic D, Nikolić N, Kostić Kravljanac L, Stanojević M, Nikolic M. Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

Carballo Méndez, Fernand, Bosnić, Predrag, Bosnic, Dragana, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Nikolic, Miroslav, "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

TY  - JOUR
AU  - Hajiboland, Roghieh
AU  - Sadeghzadeh, Noushin
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Tolra, Roser
AU  - Poschenrieder, Charlotte
AU  - Nikolic, Miroslav
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1351
AB  - Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Selenium activates components of iron acquisition machinery in oilseed rape roots
EP  - 586
IS  - 1-2
SP  - 569
VL  - 452
DO  - 10.1007/s11104-020-04599-w
ER  - 

@article{
author = "Hajiboland, Roghieh and Sadeghzadeh, Noushin and Bosnic, Dragana and Bosnić, Predrag and Tolra, Roser and Poschenrieder, Charlotte and Nikolic, Miroslav",
year = "2020",
abstract = "Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Selenium activates components of iron acquisition machinery in oilseed rape roots",
pages = "586-569",
number = "1-2",
volume = "452",
doi = "10.1007/s11104-020-04599-w"
}

Hajiboland, R., Sadeghzadeh, N., Bosnic, D., Bosnić, P., Tolra, R., Poschenrieder, C.,& Nikolic, M.. (2020). Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil
Springer, Dordrecht., 452(1-2), 569-586.
https://doi.org/10.1007/s11104-020-04599-w

Hajiboland R, Sadeghzadeh N, Bosnic D, Bosnić P, Tolra R, Poschenrieder C, Nikolic M. Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil. 2020;452(1-2):569-586.
doi:10.1007/s11104-020-04599-w .

Hajiboland, Roghieh, Sadeghzadeh, Noushin, Bosnic, Dragana, Bosnić, Predrag, Tolra, Roser, Poschenrieder, Charlotte, Nikolic, Miroslav, "Selenium activates components of iron acquisition machinery in oilseed rape roots" in Plant and Soil, 452, no. 1-2 (2020):569-586,
https://doi.org/10.1007/s11104-020-04599-w . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Nikolic, Dragana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1220
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnic, Dragana and Bosnić, Predrag and Nikolic, Dragana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnic, D., Bosnić, P., Nikolic, D., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnic D, Bosnić P, Nikolic D, Nikolic M, Samardžić JT. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnic, Dragana, Bosnić, Predrag, Nikolic, Dragana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić Kravljanac, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolic DB, Nesic S, Bosnic D, Kostić Kravljanac L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Nikolic, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1212
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnic, Dragana and Nikolic, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnic, D., Nikolic, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J. T.,& Nikolic, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnic D, Nikolic D, Timotijević G, Pavlović J, Vaculik M, Samardžić JT, Nikolic M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnic, Dragana, Nikolic, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - CONF
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardzic, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2478
AB  - The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.
PB  - University of Copenhagen, Denmark
C3  - Plant Biology Europe 2018 Conference Abstract Book
T1  - The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2478
ER  - 

@conference{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić, Ljiljana and Nikolic, Miroslav and Samardzic, Jelena",
year = "2018",
abstract = "The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.",
publisher = "University of Copenhagen, Denmark",
journal = "Plant Biology Europe 2018 Conference Abstract Book",
title = "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2478"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić, L., Nikolic, M.,& Samardzic, J.. (2018). The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2478

Nikolic DB, Nesic S, Bosnic D, Kostić L, Nikolic M, Samardzic J. The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić, Ljiljana, Nikolic, Miroslav, Samardzic, Jelena, "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution" in Plant Biology Europe 2018 Conference Abstract Book (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Jasnic, Jovana
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1106
AB  - Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Silicon mediates sodium transport and partitioning in maize under moderate salt stress
EP  - 687
SP  - 681
VL  - 155
DO  - 10.1016/j.envexpbot.2018.08.018
ER  - 

@article{
author = "Bosnić, Predrag and Bosnic, Dragana and Jasnic, Jovana and Nikolic, Miroslav",
year = "2018",
abstract = "Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Silicon mediates sodium transport and partitioning in maize under moderate salt stress",
pages = "687-681",
volume = "155",
doi = "10.1016/j.envexpbot.2018.08.018"
}

Bosnić, P., Bosnic, D., Jasnic, J.,& Nikolic, M.. (2018). Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 155, 681-687.
https://doi.org/10.1016/j.envexpbot.2018.08.018

Bosnić P, Bosnic D, Jasnic J, Nikolic M. Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany. 2018;155:681-687.
doi:10.1016/j.envexpbot.2018.08.018 .

Bosnić, Predrag, Bosnic, Dragana, Jasnic, Jovana, Nikolic, Miroslav, "Silicon mediates sodium transport and partitioning in maize under moderate salt stress" in Environmental and Experimental Botany, 155 (2018):681-687,
https://doi.org/10.1016/j.envexpbot.2018.08.018 . .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Bosnic, Dragana
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1067
AB  - Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions
EP  - 455
IS  - 1-2
SP  - 447
VL  - 419
DO  - 10.1007/s11104-017-3364-0
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Bosnic, Dragana and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2017",
abstract = "Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions",
pages = "455-447",
number = "1-2",
volume = "419",
doi = "10.1007/s11104-017-3364-0"
}

Kostić Kravljanac, L., Nikolić, N., Bosnic, D., Samardžić, J. T.,& Nikolic, M.. (2017). Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil
Springer, Dordrecht., 419(1-2), 447-455.
https://doi.org/10.1007/s11104-017-3364-0

Kostić Kravljanac L, Nikolić N, Bosnic D, Samardžić JT, Nikolic M. Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil. 2017;419(1-2):447-455.
doi:10.1007/s11104-017-3364-0 .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Bosnic, Dragana, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions" in Plant and Soil, 419, no. 1-2 (2017):447-455,
https://doi.org/10.1007/s11104-017-3364-0 . .