TY  - JOUR
AU  - Milićević, Z.
AU  - Krnjajić, Slobodan
AU  - Stević, M.
AU  - Ćirković, Jovana
AU  - Jelušić, Aleksandra
AU  - Pucarević, M.
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1549
AB  - In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.
PB  - MDPI
T2  - Agriculture (Switzerland)
T1  - Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide
IS  - 3
VL  - 12
DO  - 10.3390/agriculture12030338
ER  - 

@article{
author = "Milićević, Z. and Krnjajić, Slobodan and Stević, M. and Ćirković, Jovana and Jelušić, Aleksandra and Pucarević, M. and Popović, Tatjana",
year = "2022",
abstract = "In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.",
publisher = "MDPI",
journal = "Agriculture (Switzerland)",
title = "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide",
number = "3",
volume = "12",
doi = "10.3390/agriculture12030338"
}

Milićević, Z., Krnjajić, S., Stević, M., Ćirković, J., Jelušić, A., Pucarević, M.,& Popović, T.. (2022). Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland)
MDPI., 12(3).
https://doi.org/10.3390/agriculture12030338

Milićević Z, Krnjajić S, Stević M, Ćirković J, Jelušić A, Pucarević M, Popović T. Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland). 2022;12(3).
doi:10.3390/agriculture12030338 .

Milićević, Z., Krnjajić, Slobodan, Stević, M., Ćirković, Jovana, Jelušić, Aleksandra, Pucarević, M., Popović, Tatjana, "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide" in Agriculture (Switzerland), 12, no. 3 (2022),
https://doi.org/10.3390/agriculture12030338 . .

TY  - JOUR
AU  - Saftić-Panković, D.
AU  - Saftić-Panković, D.
AU  - Veljović-Jovanović, Sonja
AU  - Pucarević, M.
AU  - Radovanović, N.
AU  - Veljović-Jovanović, Sonja
AU  - Mijić, A.
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/162
AB  - Two near-isogenic lines of sunflower, resistant (+Pl6 gene) and susceptible (-P16 gene) to downy mildew were used in this paper. Secondary infection with a suspension of Plasmopara halstedii spores was done on the plants in the phase of first pair of leaves. In the samples taken 12 h after infection, content of phenolic compounds in methanolic extracts from frozen leaves was analysed by HPLC. POD activity was determined spectrophotmetrically and POD isoforms by isoelectrophoresis. Constitutive level of phenolic compounds and their accumulation after infection were higher in the susceptible than in the resistant line. Increased POD amount in leaf, constitutively present in NS-H-26R, was in correlation with increased guaiacol-dependent POD activity and low total phenolics contents. After infection, guaiacol- and chlorogenic acid-dependent POD activity significantly increased in both lines. Scopoletin-dependent POD activity was induced upon infection only in NS-H-26R. IEF electrophoresis revealed existance of four anionic isoforms of peroxidase in leaves of both lines. The main isoform with pI 5 was particularly intesified in the resistant line. In conclusion, scopoletin-dependent POD activity that was induced upon infection only in resistant NIL indicates a specific role of POD in coumarin metabolism that is possibly connected with the presence of Pl6 gene.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Phenolic Compounds and Peroxidases in Sunflower Near-Isogenic Lines After Downy Mildew Infection
EP  - 42
IS  - 45
SP  - 33
VL  - 29
DO  - 10.2298/hel0645033s
ER  - 

@article{
author = "Saftić-Panković, D. and Saftić-Panković, D. and Veljović-Jovanović, Sonja and Pucarević, M. and Radovanović, N. and Veljović-Jovanović, Sonja and Mijić, A.",
year = "2006",
abstract = "Two near-isogenic lines of sunflower, resistant (+Pl6 gene) and susceptible (-P16 gene) to downy mildew were used in this paper. Secondary infection with a suspension of Plasmopara halstedii spores was done on the plants in the phase of first pair of leaves. In the samples taken 12 h after infection, content of phenolic compounds in methanolic extracts from frozen leaves was analysed by HPLC. POD activity was determined spectrophotmetrically and POD isoforms by isoelectrophoresis. Constitutive level of phenolic compounds and their accumulation after infection were higher in the susceptible than in the resistant line. Increased POD amount in leaf, constitutively present in NS-H-26R, was in correlation with increased guaiacol-dependent POD activity and low total phenolics contents. After infection, guaiacol- and chlorogenic acid-dependent POD activity significantly increased in both lines. Scopoletin-dependent POD activity was induced upon infection only in NS-H-26R. IEF electrophoresis revealed existance of four anionic isoforms of peroxidase in leaves of both lines. The main isoform with pI 5 was particularly intesified in the resistant line. In conclusion, scopoletin-dependent POD activity that was induced upon infection only in resistant NIL indicates a specific role of POD in coumarin metabolism that is possibly connected with the presence of Pl6 gene.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Phenolic Compounds and Peroxidases in Sunflower Near-Isogenic Lines After Downy Mildew Infection",
pages = "42-33",
number = "45",
volume = "29",
doi = "10.2298/hel0645033s"
}

Saftić-Panković, D., Saftić-Panković, D., Veljović-Jovanović, S., Pucarević, M., Radovanović, N., Veljović-Jovanović, S.,& Mijić, A.. (2006). Phenolic Compounds and Peroxidases in Sunflower Near-Isogenic Lines After Downy Mildew Infection. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 29(45), 33-42.
https://doi.org/10.2298/hel0645033s

Saftić-Panković D, Saftić-Panković D, Veljović-Jovanović S, Pucarević M, Radovanović N, Veljović-Jovanović S, Mijić A. Phenolic Compounds and Peroxidases in Sunflower Near-Isogenic Lines After Downy Mildew Infection. in Helia. 2006;29(45):33-42.
doi:10.2298/hel0645033s .

Saftić-Panković, D., Saftić-Panković, D., Veljović-Jovanović, Sonja, Pucarević, M., Radovanović, N., Veljović-Jovanović, Sonja, Mijić, A., "Phenolic Compounds and Peroxidases in Sunflower Near-Isogenic Lines After Downy Mildew Infection" in Helia, 29, no. 45 (2006):33-42,
https://doi.org/10.2298/hel0645033s . .