TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragicević, Milan
AU  - Tešević, Vele
AU  - Stankovic-Jeremic, Jovana
AU  - Maksimović, Vuk
AU  - Cosic, Tatjana
AU  - Devrnja, Nina
AU  - Tubic, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1406
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
ER  - 

@article{
author = "Stupar, Sofija and Dragicević, Milan and Tešević, Vele and Stankovic-Jeremic, Jovana and Maksimović, Vuk and Cosic, Tatjana and Devrnja, Nina and Tubic, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172"
}

Stupar, S., Dragicević, M., Tešević, V., Stankovic-Jeremic, J., Maksimović, V., Cosic, T., Devrnja, N., Tubic, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel
MDPI, Basel., 10(1).
https://doi.org/10.3390/plants10010172

Stupar S, Dragicević M, Tešević V, Stankovic-Jeremic J, Maksimović V, Cosic T, Devrnja N, Tubic L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel. 2021;10(1).
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragicević, Milan, Tešević, Vele, Stankovic-Jeremic, Jovana, Maksimović, Vuk, Cosic, Tatjana, Devrnja, Nina, Tubic, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants-Basel, 10, no. 1 (2021),
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Calic, Dusica D
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1343
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control
EP  - 11967
IS  - 11
SP  - 11958
VL  - 27
DO  - 10.1007/s11356-020-07825-1
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Calic, Dusica D",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control",
pages = "11967-11958",
number = "11",
volume = "27",
doi = "10.1007/s11356-020-07825-1"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Calic, D. D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(11), 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Calic DD. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research. 2020;27(11):11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Calic, Dusica D, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control" in Environmental Science and Pollution Research, 27, no. 11 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Kostić, Igor
AU  - Kostic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/691
AB  - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
EP  - 122
SP  - 118
VL  - 155
DO  - 10.1016/j.scienta.2013.03.017
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Kostić, Igor and Kostic, Miroslav",
year = "2013",
abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca",
pages = "122-118",
volume = "155",
doi = "10.1016/j.scienta.2013.03.017"
}

Calic, D. D., Devrnja, N. M., Kostić, I.,& Kostic, M.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae
Elsevier, Amsterdam., 155, 118-122.
https://doi.org/10.1016/j.scienta.2013.03.017

Calic DD, Devrnja NM, Kostić I, Kostic M. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155:118-122.
doi:10.1016/j.scienta.2013.03.017 .

Calic, Dusica D, Devrnja, Nina M, Kostić, Igor, Kostic, Miroslav, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155 (2013):118-122,
https://doi.org/10.1016/j.scienta.2013.03.017 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Bohanec, Borut
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Tubic, Ljiljana
AU  - Kostić, Igor
AU  - Zdravkovic-Korac, Snežana R
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/701
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
EP  - 762
IS  - 3
SP  - 755
VL  - 27
DO  - 10.1007/s00468-012-0830-4
ER  - 

@article{
author = "Calic, Dusica D and Bohanec, Borut and Devrnja, Nina M and Milojević, Jelena D and Tubic, Ljiljana and Kostić, Igor and Zdravkovic-Korac, Snežana R",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
pages = "762-755",
number = "3",
volume = "27",
doi = "10.1007/s00468-012-0830-4"
}

Calic, D. D., Bohanec, B., Devrnja, N. M., Milojević, J. D., Tubic, L., Kostić, I.,& Zdravkovic-Korac, S. R.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 27(3), 755-762.
https://doi.org/10.1007/s00468-012-0830-4

Calic DD, Bohanec B, Devrnja NM, Milojević JD, Tubic L, Kostić I, Zdravkovic-Korac SR. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):755-762.
doi:10.1007/s00468-012-0830-4 .

Calic, Dusica D, Bohanec, Borut, Devrnja, Nina M, Milojević, Jelena D, Tubic, Ljiljana, Kostić, Igor, Zdravkovic-Korac, Snežana R, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):755-762,
https://doi.org/10.1007/s00468-012-0830-4 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Kostić, Igor
AU  - Janosević, Dusica A
AU  - Budimir, Snežana M
AU  - Zdravkovic-Korac, Snežana R
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/583
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
EP  - 1744
IS  - 12
SP  - 1741
VL  - 47
DO  - 10.21273/HORTSCI.47.12.1741
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Milojević, Jelena D and Kostić, Igor and Janosević, Dusica A and Budimir, Snežana M and Zdravkovic-Korac, Snežana R",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
pages = "1744-1741",
number = "12",
volume = "47",
doi = "10.21273/HORTSCI.47.12.1741"
}

Calic, D. D., Devrnja, N. M., Milojević, J. D., Kostić, I., Janosević, D. A., Budimir, S. M.,& Zdravkovic-Korac, S. R.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience
Amer Soc Horticultural Science, Alexandria., 47(12), 1741-1744.
https://doi.org/10.21273/HORTSCI.47.12.1741

Calic DD, Devrnja NM, Milojević JD, Kostić I, Janosević DA, Budimir SM, Zdravkovic-Korac SR. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):1741-1744.
doi:10.21273/HORTSCI.47.12.1741 .

Calic, Dusica D, Devrnja, Nina M, Milojević, Jelena D, Kostić, Igor, Janosević, Dusica A, Budimir, Snežana M, Zdravkovic-Korac, Snežana R, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):1741-1744,
https://doi.org/10.21273/HORTSCI.47.12.1741 . .