TY  - JOUR
AU  - Simonović Radosavljević, Jasna
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
AU  - Zimanyi, Laszlo
AU  - Garab, Gyozo
AU  - Steinbach, Gabor
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1427
AB  - Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts
IS  - 14
VL  - 22
DO  - 10.3390/ijms22147661
ER  - 

@article{
author = "Simonović Radosavljević, Jasna and Mitrović, Aleksandra Lj. and Radotić, Ksenija and Zimanyi, Laszlo and Garab, Gyozo and Steinbach, Gabor",
year = "2021",
abstract = "Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts",
number = "14",
volume = "22",
doi = "10.3390/ijms22147661"
}

Simonović Radosavljević, J., Mitrović, A. Lj., Radotić, K., Zimanyi, L., Garab, G.,& Steinbach, G.. (2021). Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences
MDPI, Basel., 22(14).
https://doi.org/10.3390/ijms22147661

Simonović Radosavljević J, Mitrović AL, Radotić K, Zimanyi L, Garab G, Steinbach G. Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences. 2021;22(14).
doi:10.3390/ijms22147661 .

Simonović Radosavljević, Jasna, Mitrović, Aleksandra Lj., Radotić, Ksenija, Zimanyi, Laszlo, Garab, Gyozo, Steinbach, Gabor, "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts" in International Journal of Molecular Sciences, 22, no. 14 (2021),
https://doi.org/10.3390/ijms22147661 . .