TY  - JOUR
AU  - Simonović Radosavljević, Jasna
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
AU  - Zimanyi, Laszlo
AU  - Garab, Gyozo
AU  - Steinbach, Gabor
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1427
AB  - Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts
IS  - 14
VL  - 22
DO  - 10.3390/ijms22147661
ER  - 

@article{
author = "Simonović Radosavljević, Jasna and Mitrović, Aleksandra Lj. and Radotić, Ksenija and Zimanyi, Laszlo and Garab, Gyozo and Steinbach, Gabor",
year = "2021",
abstract = "Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts",
number = "14",
volume = "22",
doi = "10.3390/ijms22147661"
}

Simonović Radosavljević, J., Mitrović, A. Lj., Radotić, K., Zimanyi, L., Garab, G.,& Steinbach, G.. (2021). Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences
MDPI, Basel., 22(14).
https://doi.org/10.3390/ijms22147661

Simonović Radosavljević J, Mitrović AL, Radotić K, Zimanyi L, Garab G, Steinbach G. Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences. 2021;22(14).
doi:10.3390/ijms22147661 .

Simonović Radosavljević, Jasna, Mitrović, Aleksandra Lj., Radotić, Ksenija, Zimanyi, Laszlo, Garab, Gyozo, Steinbach, Gabor, "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts" in International Journal of Molecular Sciences, 22, no. 14 (2021),
https://doi.org/10.3390/ijms22147661 . .

TY  - CONF
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
AU  - Devečerski, Aleksandar B
AU  - Garab, Győző
AU  - Steinbach, Gabor
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1726
AB  - Understanding of composition and connections between the building macromolecules of plant
biomass, such as cellulose, hemicellulose and lignin, is main key for their better utilization in
biofuels industry. We compared four different plant species which are abundant in the region of
the Western Balkans. We investigated the structure of the cell walls, as the main constituent of
plant biomass, isolated from branches of softwood (Picea omorika (Pancic) Purkine), hardwood
(Acer platanoides L.), maize stem (Zea mays L.) as examples of crop species, and Paulownia
tomentosa tree as a fast-growing species with a huge biomass yield. For our investigation, we
combined Fluorescence-detected linear dichroism (FDLD) method and X-ray Diffraction. We
obtained data for anisotropy and crystallography which are a base for prediction of the best and
appropriate plant species for easy deconstruction of its biomass. Our results show that Acer
branch as a hardwood shows the highest anisotropy and the lowest crystallinity compared to the
other species while Picea Omorika needles show opposite results as the lowest anisotropy and
the higher crystallinity. The results for maize show that the stems are easier for utilization than
leaves. The isolated cell walls from leaves of Paulownia tomentosa show similar results and
good correlation between anisotropy and crystallinity, thus we can conclude that this plant is
easy to use in biofuel industries.
PB  - East Sarajevo : Faculty of Agriculture, 2021
C3  - XII International Scientific Agriculture Symposium “AGROSYM 2021”
T1  - COMPARISON OF CELL WALL STRUCTURE OF DIFFERENT WESTERN BALKAN PLANT SPECIES AS A SOURCE FOR BIOFUELS
SP  - 888-892
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1726
ER  - 

@conference{
author = "Đikanović, Daniela and Radotić, Ksenija and Devečerski, Aleksandar B and Garab, Győző and Steinbach, Gabor",
year = "2021",
abstract = "Understanding of composition and connections between the building macromolecules of plant
biomass, such as cellulose, hemicellulose and lignin, is main key for their better utilization in
biofuels industry. We compared four different plant species which are abundant in the region of
the Western Balkans. We investigated the structure of the cell walls, as the main constituent of
plant biomass, isolated from branches of softwood (Picea omorika (Pancic) Purkine), hardwood
(Acer platanoides L.), maize stem (Zea mays L.) as examples of crop species, and Paulownia
tomentosa tree as a fast-growing species with a huge biomass yield. For our investigation, we
combined Fluorescence-detected linear dichroism (FDLD) method and X-ray Diffraction. We
obtained data for anisotropy and crystallography which are a base for prediction of the best and
appropriate plant species for easy deconstruction of its biomass. Our results show that Acer
branch as a hardwood shows the highest anisotropy and the lowest crystallinity compared to the
other species while Picea Omorika needles show opposite results as the lowest anisotropy and
the higher crystallinity. The results for maize show that the stems are easier for utilization than
leaves. The isolated cell walls from leaves of Paulownia tomentosa show similar results and
good correlation between anisotropy and crystallinity, thus we can conclude that this plant is
easy to use in biofuel industries.",
publisher = "East Sarajevo : Faculty of Agriculture, 2021",
journal = "XII International Scientific Agriculture Symposium “AGROSYM 2021”",
title = "COMPARISON OF CELL WALL STRUCTURE OF DIFFERENT WESTERN BALKAN PLANT SPECIES AS A SOURCE FOR BIOFUELS",
pages = "888-892",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1726"
}

Đikanović, D., Radotić, K., Devečerski, A. B., Garab, G.,& Steinbach, G.. (2021). COMPARISON OF CELL WALL STRUCTURE OF DIFFERENT WESTERN BALKAN PLANT SPECIES AS A SOURCE FOR BIOFUELS. in XII International Scientific Agriculture Symposium “AGROSYM 2021”
East Sarajevo : Faculty of Agriculture, 2021., 888-892.
https://hdl.handle.net/21.15107/rcub_rimsi_1726

Đikanović D, Radotić K, Devečerski AB, Garab G, Steinbach G. COMPARISON OF CELL WALL STRUCTURE OF DIFFERENT WESTERN BALKAN PLANT SPECIES AS A SOURCE FOR BIOFUELS. in XII International Scientific Agriculture Symposium “AGROSYM 2021”. 2021;:888-892.
https://hdl.handle.net/21.15107/rcub_rimsi_1726 .

Đikanović, Daniela, Radotić, Ksenija, Devečerski, Aleksandar B, Garab, Győző, Steinbach, Gabor, "COMPARISON OF CELL WALL STRUCTURE OF DIFFERENT WESTERN BALKAN PLANT SPECIES AS A SOURCE FOR BIOFUELS" in XII International Scientific Agriculture Symposium “AGROSYM 2021” (2021):888-892,
https://hdl.handle.net/21.15107/rcub_rimsi_1726 .

TY  - GEN
AU  - Radotić, Ksenija
AU  - Simonović Radosavljević, Jasna
AU  - Donaldson, Lloyd
AU  - Garab, Gyozo
AU  - Dudits, D.
AU  - Steinbach, Gabor
AU  - Mitrović, Aleksandra Lj.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1232
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Gravitropic response in woody species: role of stem structural anisotropy
EP  - S102
SP  - S102
VL  - 48
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1232
ER  - 

@misc{
author = "Radotić, Ksenija and Simonović Radosavljević, Jasna and Donaldson, Lloyd and Garab, Gyozo and Dudits, D. and Steinbach, Gabor and Mitrović, Aleksandra Lj.",
year = "2019",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Gravitropic response in woody species: role of stem structural anisotropy",
pages = "S102-S102",
volume = "48",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1232"
}

Radotić, K., Simonović Radosavljević, J., Donaldson, L., Garab, G., Dudits, D., Steinbach, G.,& Mitrović, A. Lj.. (2019). Gravitropic response in woody species: role of stem structural anisotropy. in European Biophysics Journal With Biophysics Letters
Springer, New York., 48, S102-S102.
https://hdl.handle.net/21.15107/rcub_rimsi_1232

Radotić K, Simonović Radosavljević J, Donaldson L, Garab G, Dudits D, Steinbach G, Mitrović AL. Gravitropic response in woody species: role of stem structural anisotropy. in European Biophysics Journal With Biophysics Letters. 2019;48:S102-S102.
https://hdl.handle.net/21.15107/rcub_rimsi_1232 .

Radotić, Ksenija, Simonović Radosavljević, Jasna, Donaldson, Lloyd, Garab, Gyozo, Dudits, D., Steinbach, Gabor, Mitrović, Aleksandra Lj., "Gravitropic response in woody species: role of stem structural anisotropy" in European Biophysics Journal With Biophysics Letters, 48 (2019):S102-S102,
https://hdl.handle.net/21.15107/rcub_rimsi_1232 .

TY  - JOUR
AU  - Nedzved, Aleksander
AU  - Mitrović, Aleksandra Lj.
AU  - Savić, Aleksandar G
AU  - Mutavdžić, Dragosav
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, Gyozo
AU  - Starovoytov, Valery
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1116
AB  - Measurements of various anatomical characteristics of wood cells are of great importance in research of wood structure, either for the evaluation of environmental influences or for estimation of wood quality. We present and test an automatic image processing morphometric method for the analysis of tracheid double wall thickness. A new algorithm of image analysis was developed. It uses morphological processing of structural elements with the different orientations from distance maps to analyze tracheid double wall thickness distribution separately for radial walls, tangential walls, and cell corners. For testing the performance of the method, we used confocal laser scanning microscopy images of stem cross-sections of juvenile Picea omorika trees exposed to long-term static bending. As a response to mechanical stress, conifers form compression wood (CW), which occurs in a range of gradations from near normal wood (NW) to severe CW. However, visual detection of compression wood severity, more precisely the determination of mild CW, is difficult. One of the anatomic features that characterize CW is increased wall thickness. After testing proposed automatic image processing morphometric method for the analysis of tracheid double wall thickness separately for radial walls, tangential walls and cell corners, combined with statistical analysis, we could suggest it as a tool for estimation of compression wood severity, or for estimation and gradation of changes in tracheid cell wall thickness as a response to environmental influences during growth and developmental process.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending
EP  - 1356
IS  - 5
SP  - 1347
VL  - 32
DO  - 10.1007/s00468-018-1716-x
ER  - 

@article{
author = "Nedzved, Aleksander and Mitrović, Aleksandra Lj. and Savić, Aleksandar G and Mutavdžić, Dragosav and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, Gyozo and Starovoytov, Valery and Radotić, Ksenija",
year = "2018",
abstract = "Measurements of various anatomical characteristics of wood cells are of great importance in research of wood structure, either for the evaluation of environmental influences or for estimation of wood quality. We present and test an automatic image processing morphometric method for the analysis of tracheid double wall thickness. A new algorithm of image analysis was developed. It uses morphological processing of structural elements with the different orientations from distance maps to analyze tracheid double wall thickness distribution separately for radial walls, tangential walls, and cell corners. For testing the performance of the method, we used confocal laser scanning microscopy images of stem cross-sections of juvenile Picea omorika trees exposed to long-term static bending. As a response to mechanical stress, conifers form compression wood (CW), which occurs in a range of gradations from near normal wood (NW) to severe CW. However, visual detection of compression wood severity, more precisely the determination of mild CW, is difficult. One of the anatomic features that characterize CW is increased wall thickness. After testing proposed automatic image processing morphometric method for the analysis of tracheid double wall thickness separately for radial walls, tangential walls and cell corners, combined with statistical analysis, we could suggest it as a tool for estimation of compression wood severity, or for estimation and gradation of changes in tracheid cell wall thickness as a response to environmental influences during growth and developmental process.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending",
pages = "1356-1347",
number = "5",
volume = "32",
doi = "10.1007/s00468-018-1716-x"
}

Nedzved, A., Mitrović, A. Lj., Savić, A. G., Mutavdžić, D., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G., Starovoytov, V.,& Radotić, K.. (2018). Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 32(5), 1347-1356.
https://doi.org/10.1007/s00468-018-1716-x

Nedzved A, Mitrović AL, Savić AG, Mutavdžić D, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Starovoytov V, Radotić K. Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending. in Trees-Structure and Function. 2018;32(5):1347-1356.
doi:10.1007/s00468-018-1716-x .

Nedzved, Aleksander, Mitrović, Aleksandra Lj., Savić, Aleksandar G, Mutavdžić, Dragosav, Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, Gyozo, Starovoytov, Valery, Radotić, Ksenija, "Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending" in Trees-Structure and Function, 32, no. 5 (2018):1347-1356,
https://doi.org/10.1007/s00468-018-1716-x . .

TY  - JOUR
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Mitrović, Aleksandra Lj.
AU  - Steinbach, Gabor
AU  - Mouille, Gregory
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Janosević, Dusica
AU  - Vuković, Marina
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1057
AB  - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Parenchyma cell wall structure in twining stem of Dioscorea balcanica
EP  - 4669
IS  - 11
SP  - 4653
VL  - 24
DO  - 10.1007/s10570-017-1460-1
ER  - 

@article{
author = "Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Mitrović, Aleksandra Lj. and Steinbach, Gabor and Mouille, Gregory and Tufegdžić, Srđan J. and Maksimović, Vuk and Mutavdžić, Dragosav and Janosević, Dusica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2017",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Parenchyma cell wall structure in twining stem of Dioscorea balcanica",
pages = "4669-4653",
number = "11",
volume = "24",
doi = "10.1007/s10570-017-1460-1"
}

Simonović Radosavljević, J., Bogdanović Pristov, J., Mitrović, A. Lj., Steinbach, G., Mouille, G., Tufegdžić, S. J., Maksimović, V., Mutavdžić, D., Janosević, D., Vuković, M., Garab, G.,& Radotić, K.. (2017). Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose
Springer, Dordrecht., 24(11), 4653-4669.
https://doi.org/10.1007/s10570-017-1460-1

Simonović Radosavljević J, Bogdanović Pristov J, Mitrović AL, Steinbach G, Mouille G, Tufegdžić SJ, Maksimović V, Mutavdžić D, Janosević D, Vuković M, Garab G, Radotić K. Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose. 2017;24(11):4653-4669.
doi:10.1007/s10570-017-1460-1 .

Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Mitrović, Aleksandra Lj., Steinbach, Gabor, Mouille, Gregory, Tufegdžić, Srđan J., Maksimović, Vuk, Mutavdžić, Dragosav, Janosević, Dusica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Parenchyma cell wall structure in twining stem of Dioscorea balcanica" in Cellulose, 24, no. 11 (2017):4653-4669,
https://doi.org/10.1007/s10570-017-1460-1 . .

TY  - JOUR
AU  - Savić, Aleksandar G
AU  - Mitrović, Aleksandra Lj.
AU  - Donaldson, Lloyd
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/989
AB  - Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.
PB  - Cambridge Univ Press, New York
T2  - Microscopy and Microanalysis
T1  - Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity
EP  - 367
IS  - 2
SP  - 361
VL  - 22
DO  - 10.1017/S143192761600009X
ER  - 

@article{
author = "Savić, Aleksandar G and Mitrović, Aleksandra Lj. and Donaldson, Lloyd and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, Gyozo and Radotić, Ksenija",
year = "2016",
abstract = "Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.",
publisher = "Cambridge Univ Press, New York",
journal = "Microscopy and Microanalysis",
title = "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity",
pages = "367-361",
number = "2",
volume = "22",
doi = "10.1017/S143192761600009X"
}

Savić, A. G., Mitrović, A. Lj., Donaldson, L., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G.,& Radotić, K.. (2016). Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis
Cambridge Univ Press, New York., 22(2), 361-367.
https://doi.org/10.1017/S143192761600009X

Savić AG, Mitrović AL, Donaldson L, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Radotić K. Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis. 2016;22(2):361-367.
doi:10.1017/S143192761600009X .

Savić, Aleksandar G, Mitrović, Aleksandra Lj., Donaldson, Lloyd, Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, Gyozo, Radotić, Ksenija, "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity" in Microscopy and Microanalysis, 22, no. 2 (2016):361-367,
https://doi.org/10.1017/S143192761600009X . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Devečerski, Aleksandar B
AU  - Steinbach, Gabor
AU  - Simonović Radosavljević, Jasna
AU  - Matović, Branko
AU  - Garab, Gyozo
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1021
AB  - Interactions between macromolecules in the cell walls of different plant origin were compared, namely spruce wood (Picea omorika (PaniA double dagger) PurkiAe) as an example of softwood, maple wood (Acer platanoides L.) as a hardwood and maize stems (Zea mays L.) as a herbaceous plant from the grass family and widely used agricultural plant. Interactions of macromolecules in isolated cell walls from the three species were compared by using Fourier transform infrared spectroscopy, X-ray diffraction and fluorescence spectroscopy. Linear dichroism of the cell walls was observed by using differential polarization laser scanning microscope (DP-LSM), which provides information of macromolecular order. This method has not previously been used for comparison of the cell walls of various plant origins. It was shown that the maize cell walls have higher amount of hydrogen bonds that lead to more regular packing of cellulose molecules, simpler structure of lignin, and a higher crystallinity of the cell wall in relation to the walls of woody plants. DP-LSM and fluorescence spectroscopy results indicate that maize has simpler and more ordered structure than both woody species. The results of this work provide new data for comparison of the cell wall properties that may be important for selection of appropriate plant for possible applications as a source of biomass. This may be a contribution to the development of efficient deconstruction and separation technologies that enable release of sugar and aromatic compounds from the cell wall macromolecular structure.
PB  - Springer, New York
T2  - Wood Science and Technology
T1  - Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction
EP  - 566
IS  - 3
SP  - 547
VL  - 50
DO  - 10.1007/s00226-015-0792-y
ER  - 

@article{
author = "Đikanović, Daniela and Devečerski, Aleksandar B and Steinbach, Gabor and Simonović Radosavljević, Jasna and Matović, Branko and Garab, Gyozo and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2016",
abstract = "Interactions between macromolecules in the cell walls of different plant origin were compared, namely spruce wood (Picea omorika (PaniA double dagger) PurkiAe) as an example of softwood, maple wood (Acer platanoides L.) as a hardwood and maize stems (Zea mays L.) as a herbaceous plant from the grass family and widely used agricultural plant. Interactions of macromolecules in isolated cell walls from the three species were compared by using Fourier transform infrared spectroscopy, X-ray diffraction and fluorescence spectroscopy. Linear dichroism of the cell walls was observed by using differential polarization laser scanning microscope (DP-LSM), which provides information of macromolecular order. This method has not previously been used for comparison of the cell walls of various plant origins. It was shown that the maize cell walls have higher amount of hydrogen bonds that lead to more regular packing of cellulose molecules, simpler structure of lignin, and a higher crystallinity of the cell wall in relation to the walls of woody plants. DP-LSM and fluorescence spectroscopy results indicate that maize has simpler and more ordered structure than both woody species. The results of this work provide new data for comparison of the cell wall properties that may be important for selection of appropriate plant for possible applications as a source of biomass. This may be a contribution to the development of efficient deconstruction and separation technologies that enable release of sugar and aromatic compounds from the cell wall macromolecular structure.",
publisher = "Springer, New York",
journal = "Wood Science and Technology",
title = "Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction",
pages = "566-547",
number = "3",
volume = "50",
doi = "10.1007/s00226-015-0792-y"
}

Đikanović, D., Devečerski, A. B., Steinbach, G., Simonović Radosavljević, J., Matović, B., Garab, G., Kalauzi, A.,& Radotić, K.. (2016). Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction. in Wood Science and Technology
Springer, New York., 50(3), 547-566.
https://doi.org/10.1007/s00226-015-0792-y

Đikanović D, Devečerski AB, Steinbach G, Simonović Radosavljević J, Matović B, Garab G, Kalauzi A, Radotić K. Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction. in Wood Science and Technology. 2016;50(3):547-566.
doi:10.1007/s00226-015-0792-y .

Đikanović, Daniela, Devečerski, Aleksandar B, Steinbach, Gabor, Simonović Radosavljević, Jasna, Matović, Branko, Garab, Gyozo, Kalauzi, Aleksandar, Radotić, Ksenija, "Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction" in Wood Science and Technology, 50, no. 3 (2016):547-566,
https://doi.org/10.1007/s00226-015-0792-y . .

TY  - CONF
AU  - Savić, Aleksandar
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, G
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2881
PB  - Departmant of Biology and Ecology, Faculty of Science and Mathematics, University of Niš
PB  - Biological Society "Dr Sava Petrović" Niš
C3  - 11th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions
T1  - Fluorescence detected linear dichroism of cellulose fibers in Picea omorika stems as a measure of mechanical stress
SP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2881
ER  - 

@conference{
author = "Savić, Aleksandar and Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, G and Radotić, Ksenija",
year = "2013",
publisher = "Departmant of Biology and Ecology, Faculty of Science and Mathematics, University of Niš, Biological Society "Dr Sava Petrović" Niš",
journal = "11th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions",
title = "Fluorescence detected linear dichroism of cellulose fibers in Picea omorika stems as a measure of mechanical stress",
pages = "120",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2881"
}

Savić, A., Mitrović, A. Lj., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G.,& Radotić, K.. (2013). Fluorescence detected linear dichroism of cellulose fibers in Picea omorika stems as a measure of mechanical stress. in 11th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions
Departmant of Biology and Ecology, Faculty of Science and Mathematics, University of Niš., 120.
https://hdl.handle.net/21.15107/rcub_rimsi_2881

Savić A, Mitrović AL, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Radotić K. Fluorescence detected linear dichroism of cellulose fibers in Picea omorika stems as a measure of mechanical stress. in 11th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions. 2013;:120.
https://hdl.handle.net/21.15107/rcub_rimsi_2881 .

Savić, Aleksandar, Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, G, Radotić, Ksenija, "Fluorescence detected linear dichroism of cellulose fibers in Picea omorika stems as a measure of mechanical stress" in 11th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions (2013):120,
https://hdl.handle.net/21.15107/rcub_rimsi_2881 .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Roduit, Charles
AU  - Simonović Radosavljević, Jasna
AU  - Hornitschek, Patricia
AU  - Fankhauser, Christian
AU  - Mutavdžić, Dragosav
AU  - Steinbach, Gabor
AU  - Dietler, Giovanni
AU  - Kasas, Sandor
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/594
AB  - Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.
PB  - Cell Press, Cambridge
T2  - Biophysical Journal
T1  - Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth
EP  - 394
IS  - 3
SP  - 386
VL  - 103
DO  - 10.1016/j.bpj.2012.06.046
ER  - 

@article{
author = "Radotić, Ksenija and Roduit, Charles and Simonović Radosavljević, Jasna and Hornitschek, Patricia and Fankhauser, Christian and Mutavdžić, Dragosav and Steinbach, Gabor and Dietler, Giovanni and Kasas, Sandor",
year = "2012",
abstract = "Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.",
publisher = "Cell Press, Cambridge",
journal = "Biophysical Journal",
title = "Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth",
pages = "394-386",
number = "3",
volume = "103",
doi = "10.1016/j.bpj.2012.06.046"
}

Radotić, K., Roduit, C., Simonović Radosavljević, J., Hornitschek, P., Fankhauser, C., Mutavdžić, D., Steinbach, G., Dietler, G.,& Kasas, S.. (2012). Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth. in Biophysical Journal
Cell Press, Cambridge., 103(3), 386-394.
https://doi.org/10.1016/j.bpj.2012.06.046

Radotić K, Roduit C, Simonović Radosavljević J, Hornitschek P, Fankhauser C, Mutavdžić D, Steinbach G, Dietler G, Kasas S. Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth. in Biophysical Journal. 2012;103(3):386-394.
doi:10.1016/j.bpj.2012.06.046 .

Radotić, Ksenija, Roduit, Charles, Simonović Radosavljević, Jasna, Hornitschek, Patricia, Fankhauser, Christian, Mutavdžić, Dragosav, Steinbach, Gabor, Dietler, Giovanni, Kasas, Sandor, "Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth" in Biophysical Journal, 103, no. 3 (2012):386-394,
https://doi.org/10.1016/j.bpj.2012.06.046 . .

TY  - CONF
AU  - Simonović, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Mitrović, Aleksandra Lj.
AU  - Steinbach, Gabor
AU  - Mouille, Gregory
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3039
C3  - Hierarchical structure and mechanical characterization of wood, Helsinki, Finland
T1  - Cell wall linear dichroism in the Dioscorea balcanica stems sections
EP  - 30
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3039
ER  - 

@conference{
author = "Simonović, Jasna and Bogdanović Pristov, Jelena and Mitrović, Aleksandra Lj. and Steinbach, Gabor and Mouille, Gregory and Garab, Gyozo and Radotić, Ksenija",
year = "2011",
journal = "Hierarchical structure and mechanical characterization of wood, Helsinki, Finland",
title = "Cell wall linear dichroism in the Dioscorea balcanica stems sections",
pages = "30-29",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3039"
}

Simonović, J., Bogdanović Pristov, J., Mitrović, A. Lj., Steinbach, G., Mouille, G., Garab, G.,& Radotić, K.. (2011). Cell wall linear dichroism in the Dioscorea balcanica stems sections. in Hierarchical structure and mechanical characterization of wood, Helsinki, Finland, 29-30.
https://hdl.handle.net/21.15107/rcub_rimsi_3039

Simonović J, Bogdanović Pristov J, Mitrović AL, Steinbach G, Mouille G, Garab G, Radotić K. Cell wall linear dichroism in the Dioscorea balcanica stems sections. in Hierarchical structure and mechanical characterization of wood, Helsinki, Finland. 2011;:29-30.
https://hdl.handle.net/21.15107/rcub_rimsi_3039 .

Simonović, Jasna, Bogdanović Pristov, Jelena, Mitrović, Aleksandra Lj., Steinbach, Gabor, Mouille, Gregory, Garab, Gyozo, Radotić, Ksenija, "Cell wall linear dichroism in the Dioscorea balcanica stems sections" in Hierarchical structure and mechanical characterization of wood, Helsinki, Finland (2011):29-30,
https://hdl.handle.net/21.15107/rcub_rimsi_3039 .