TY  - JOUR
AU  - Živanović, Bojana
AU  - Vidović, Marija
AU  - Milić Komić, Sonja
AU  - Jovanović, Ljubinko
AU  - Kolarz, Predrag
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1066
AB  - Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Agriculture and Forestry
T1  - Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates
EP  - +
IS  - 2
SP  - 113
VL  - 41
DO  - 10.3906/tar-1612-56
ER  - 

@article{
author = "Živanović, Bojana and Vidović, Marija and Milić Komić, Sonja and Jovanović, Ljubinko and Kolarz, Predrag and Morina, Filis and Veljović-Jovanović, Sonja",
year = "2017",
abstract = "Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and beta-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Agriculture and Forestry",
title = "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates",
pages = "+-113",
number = "2",
volume = "41",
doi = "10.3906/tar-1612-56"
}

Živanović, B., Vidović, M., Milić Komić, S., Jovanović, L., Kolarz, P., Morina, F.,& Veljović-Jovanović, S.. (2017). Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry
Tubitak Scientific & Technical Research Council Turkey, Ankara., 41(2), 113-+.
https://doi.org/10.3906/tar-1612-56

Živanović B, Vidović M, Milić Komić S, Jovanović L, Kolarz P, Morina F, Veljović-Jovanović S. Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates. in Turkish Journal of Agriculture and Forestry. 2017;41(2):113-+.
doi:10.3906/tar-1612-56 .

Živanović, Bojana, Vidović, Marija, Milić Komić, Sonja, Jovanović, Ljubinko, Kolarz, Predrag, Morina, Filis, Veljović-Jovanović, Sonja, "Contents of phenolics and carotenoids in tomato grown under polytunnels with different UV-transmission rates" in Turkish Journal of Agriculture and Forestry, 41, no. 2 (2017):113-+,
https://doi.org/10.3906/tar-1612-56 . .

TY  - JOUR
AU  - Morina, Filis
AU  - Jovanović, Ljubinko
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
AU  - Smith, J. Andrew C.
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/978
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas
EP  - 10021
EP  - metal contaminated soil / phytoremediation / Zinc / Copper / Verbascum populations
IS  - 10
SP  - 10021
VL  - 23
DO  - 10.1007/s11356-016-6658-5
ER  - 

@article{
author = "Morina, Filis and Jovanović, Ljubinko and Prokic, Ljiljana and Veljović-Jovanović, Sonja and Smith, J. Andrew C.",
year = "2016",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas",
pages = "10021-metal contaminated soil / phytoremediation / Zinc / Copper / Verbascum populations-10021",
number = "10",
volume = "23",
doi = "10.1007/s11356-016-6658-5"
}

Morina, F., Jovanović, L., Prokic, L., Veljović-Jovanović, S.,& Smith, J. A. C.. (2016). Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(10), 10021-10021.
https://doi.org/10.1007/s11356-016-6658-5

Morina F, Jovanović L, Prokic L, Veljović-Jovanović S, Smith JAC. Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research. 2016;23(10):10021-10021.
doi:10.1007/s11356-016-6658-5 .

Morina, Filis, Jovanović, Ljubinko, Prokic, Ljiljana, Veljović-Jovanović, Sonja, Smith, J. Andrew C., "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas" in Environmental Science and Pollution Research, 23, no. 10 (2016):10021-10021,
https://doi.org/10.1007/s11356-016-6658-5 . .

TY  - JOUR
AU  - Morina, Filis
AU  - Jovanović, Ljubinko
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/934
AB  - Metal contamination represents a strong selective pressure favoring tolerant genotypes and leading to differentiation between plant populations. We investigated the adaptive capacity of early-colonizer species of Verbascum recently exposed to Zn- and Cu-contaminated soils (10-20 years). Two Verbascum thapsus L. populations from uncontaminated sites (NMET1, NMET2), one V. thapsus from a zinc-contaminated site (MET1), and a Verbascum lychnitis population from an open-cast copper mine (MET2) were exposed to elevated Zn or Cu in hydroponic culture under glasshouse conditions. MET populations showed considerably higher tolerance to both Zn and Cu than NMET populations as assessed by measurements of growth and net photosynthesis, yet they accumulated higher tissue Zn concentrations in the shoot. Abscisic acid (ABA) concentration increased with Zn and Cu treatment in the NMET populations, which was correlated to stomatal closure, decrease of net photosynthesis, and nutritional imbalance, indicative of interference with xylem loading and divalent-cation homeostasis. At the cellular level, the sensitivity of NMET2 to Zn and Cu was reflected in significant metal-induced ROS accumulation and ion leakage from roots as well as strong induction of peroxidase activity (POD, EC 1.11.1.7), while Zn had no significant effect on ABA concentration and POD activity in MET1. Interestingly, MET2 had constitutively higher root ABA concentration and POD activity. We propose that ABA distribution between shoots and roots could represent an adaptive mechanism for maintaining low ABA levels and unaffected stomatal conductance. The results show that metal tolerance can occur in Verbascum populations after relatively short time of exposure to metal-contaminated soil, indicating their potential use for phytostabilization.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas
EP  - 10020
IS  - 10
SP  - 10005
VL  - 23
DO  - 10.1007/s11356-016-6177-4
ER  - 

@article{
author = "Morina, Filis and Jovanović, Ljubinko and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Metal contamination represents a strong selective pressure favoring tolerant genotypes and leading to differentiation between plant populations. We investigated the adaptive capacity of early-colonizer species of Verbascum recently exposed to Zn- and Cu-contaminated soils (10-20 years). Two Verbascum thapsus L. populations from uncontaminated sites (NMET1, NMET2), one V. thapsus from a zinc-contaminated site (MET1), and a Verbascum lychnitis population from an open-cast copper mine (MET2) were exposed to elevated Zn or Cu in hydroponic culture under glasshouse conditions. MET populations showed considerably higher tolerance to both Zn and Cu than NMET populations as assessed by measurements of growth and net photosynthesis, yet they accumulated higher tissue Zn concentrations in the shoot. Abscisic acid (ABA) concentration increased with Zn and Cu treatment in the NMET populations, which was correlated to stomatal closure, decrease of net photosynthesis, and nutritional imbalance, indicative of interference with xylem loading and divalent-cation homeostasis. At the cellular level, the sensitivity of NMET2 to Zn and Cu was reflected in significant metal-induced ROS accumulation and ion leakage from roots as well as strong induction of peroxidase activity (POD, EC 1.11.1.7), while Zn had no significant effect on ABA concentration and POD activity in MET1. Interestingly, MET2 had constitutively higher root ABA concentration and POD activity. We propose that ABA distribution between shoots and roots could represent an adaptive mechanism for maintaining low ABA levels and unaffected stomatal conductance. The results show that metal tolerance can occur in Verbascum populations after relatively short time of exposure to metal-contaminated soil, indicating their potential use for phytostabilization.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas",
pages = "10020-10005",
number = "10",
volume = "23",
doi = "10.1007/s11356-016-6177-4"
}

Morina, F., Jovanović, L., Prokic, L.,& Veljović-Jovanović, S.. (2016). Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(10), 10005-10020.
https://doi.org/10.1007/s11356-016-6177-4

Morina F, Jovanović L, Prokic L, Veljović-Jovanović S. Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research. 2016;23(10):10005-10020.
doi:10.1007/s11356-016-6177-4 .

Morina, Filis, Jovanović, Ljubinko, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas" in Environmental Science and Pollution Research, 23, no. 10 (2016):10005-10020,
https://doi.org/10.1007/s11356-016-6177-4 . .

TY  - JOUR
AU  - Kukavica, Biljana
AU  - Morina, Filis
AU  - Janjic, Nina
AU  - Boroja, Mirela
AU  - Jovanović, Ljubinko
AU  - Veljović-Jovanović, Sonja
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/638
AB  - The effects of hyperalkaline, thermo-mineral water from Slatina on the morphology and anatomy of pea plants (Pisum sativum L.), were examined after eleven days of treatment with a mixture of tap water and Slatina water in 3:1 (T1) and 1:1 ratios (T2). Complete growth arrest of seedlings was observed in the Slatina water (T3). The alkalinity of external media was recovered to pH 8 within four days only in T1 and T2. Analysis of morphological parameters (the length of the main root, root application zone, number of lateral roots) indicated that the thermo-mineral water either promoted (T1) or inhibited (T2) the formation of lateral roots and plant growth. Comparative histological and anatomical analyses showed that inhibition of lateral roots was accompanied by an increase in the xylem and phloem. These changes in root morphology were accompanied by an increase in the activity of superoxide dismutase (SOD: E.C. 1.15.1.1) and peroxidase (POD: E.C 1.1.1.17) in the soluble fraction, whereas the activities of ascorbate oxidase (AAO: E.C. 1.10.3.3) bound to the cell wall and ionic POD decreased. The lower ratio of Slatina water in the hydroponic solution contributed to a more developed mesophyll with significantly higher AAO activity in the leaves and the induction of ionic POD isoforms. Besides alkalinity and excess NaCl, we suggest that a specific combination of metals (e.g. Ca and Mg) might be responsible for subtle changes in the cell area and xylem development, leading to dramatic changes in root anatomy.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effects of mixed saline and alkaline stress on the morphology and anatomy of pisum sativum l.: the role of peroxidase and ascorbate oxidase in growth regulation
EP  - 278
IS  - 1
SP  - 265
VL  - 65
DO  - 10.2298/ABS1301265K
ER  - 

@article{
author = "Kukavica, Biljana and Morina, Filis and Janjic, Nina and Boroja, Mirela and Jovanović, Ljubinko and Veljović-Jovanović, Sonja",
year = "2013",
abstract = "The effects of hyperalkaline, thermo-mineral water from Slatina on the morphology and anatomy of pea plants (Pisum sativum L.), were examined after eleven days of treatment with a mixture of tap water and Slatina water in 3:1 (T1) and 1:1 ratios (T2). Complete growth arrest of seedlings was observed in the Slatina water (T3). The alkalinity of external media was recovered to pH 8 within four days only in T1 and T2. Analysis of morphological parameters (the length of the main root, root application zone, number of lateral roots) indicated that the thermo-mineral water either promoted (T1) or inhibited (T2) the formation of lateral roots and plant growth. Comparative histological and anatomical analyses showed that inhibition of lateral roots was accompanied by an increase in the xylem and phloem. These changes in root morphology were accompanied by an increase in the activity of superoxide dismutase (SOD: E.C. 1.15.1.1) and peroxidase (POD: E.C 1.1.1.17) in the soluble fraction, whereas the activities of ascorbate oxidase (AAO: E.C. 1.10.3.3) bound to the cell wall and ionic POD decreased. The lower ratio of Slatina water in the hydroponic solution contributed to a more developed mesophyll with significantly higher AAO activity in the leaves and the induction of ionic POD isoforms. Besides alkalinity and excess NaCl, we suggest that a specific combination of metals (e.g. Ca and Mg) might be responsible for subtle changes in the cell area and xylem development, leading to dramatic changes in root anatomy.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effects of mixed saline and alkaline stress on the morphology and anatomy of pisum sativum l.: the role of peroxidase and ascorbate oxidase in growth regulation",
pages = "278-265",
number = "1",
volume = "65",
doi = "10.2298/ABS1301265K"
}

Kukavica, B., Morina, F., Janjic, N., Boroja, M., Jovanović, L.,& Veljović-Jovanović, S.. (2013). Effects of mixed saline and alkaline stress on the morphology and anatomy of pisum sativum l.: the role of peroxidase and ascorbate oxidase in growth regulation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(1), 265-278.
https://doi.org/10.2298/ABS1301265K

Kukavica B, Morina F, Janjic N, Boroja M, Jovanović L, Veljović-Jovanović S. Effects of mixed saline and alkaline stress on the morphology and anatomy of pisum sativum l.: the role of peroxidase and ascorbate oxidase in growth regulation. in Archives of Biological Sciences. 2013;65(1):265-278.
doi:10.2298/ABS1301265K .

Kukavica, Biljana, Morina, Filis, Janjic, Nina, Boroja, Mirela, Jovanović, Ljubinko, Veljović-Jovanović, Sonja, "Effects of mixed saline and alkaline stress on the morphology and anatomy of pisum sativum l.: the role of peroxidase and ascorbate oxidase in growth regulation" in Archives of Biological Sciences, 65, no. 1 (2013):265-278,
https://doi.org/10.2298/ABS1301265K . .

TY  - JOUR
AU  - Jarić, Ivan
AU  - Višnjić Jeftić, Željka
AU  - Cvijanović, Gorčin
AU  - Gačić, Zoran
AU  - Jovanović, Ljubinko
AU  - Skorić, Stefan
AU  - Lenhardt, Mirjana
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/446
AB  - The accumulation of heavy metals in fish has been extensively studied and well documented. However, the research has been mainly focused on the muscle tissue, while the distribution patterns among other tissues, such as liver and gills, have been mostly neglected. Within the present study, the concentrations of Ag, Al, As, B, Ba, Cd, Co, Cr, Cu, Fe. Mn, Mo, Ni, Pb, Se, Sr, Zn and Li in the muscle, gills, liver and intestine of the sterlet (Acipenser ruthenus) from the Danube River have been assessed by using the inductively-coupled plasma optical spectrometry. The analysis has revealed a high degree of differential accumulation of the studied heavy metals and trace elements in the tissues of the Danube sterlet. Liver appeared to be the main heavy metal storage tissue, while the lowest levels of analyzed metals were found in muscles. Heavy metal concentrations in the muscle were at acceptable levels for human consumption, except partly for cadmium. We conclude that, in order to enable a more efficient control of contaminants in fish products, there is an apparent need to resolve the problem of lack of legal limits for many metals in European legislation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Microchemical Journal
T1  - Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of sterlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES
EP  - 81
IS  - 1
SP  - 77
VL  - 98
DO  - 10.1016/j.microc.2010.11.008
ER  - 

@article{
author = "Jarić, Ivan and Višnjić Jeftić, Željka and Cvijanović, Gorčin and Gačić, Zoran and Jovanović, Ljubinko and Skorić, Stefan and Lenhardt, Mirjana",
year = "2011",
abstract = "The accumulation of heavy metals in fish has been extensively studied and well documented. However, the research has been mainly focused on the muscle tissue, while the distribution patterns among other tissues, such as liver and gills, have been mostly neglected. Within the present study, the concentrations of Ag, Al, As, B, Ba, Cd, Co, Cr, Cu, Fe. Mn, Mo, Ni, Pb, Se, Sr, Zn and Li in the muscle, gills, liver and intestine of the sterlet (Acipenser ruthenus) from the Danube River have been assessed by using the inductively-coupled plasma optical spectrometry. The analysis has revealed a high degree of differential accumulation of the studied heavy metals and trace elements in the tissues of the Danube sterlet. Liver appeared to be the main heavy metal storage tissue, while the lowest levels of analyzed metals were found in muscles. Heavy metal concentrations in the muscle were at acceptable levels for human consumption, except partly for cadmium. We conclude that, in order to enable a more efficient control of contaminants in fish products, there is an apparent need to resolve the problem of lack of legal limits for many metals in European legislation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Microchemical Journal",
title = "Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of sterlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES",
pages = "81-77",
number = "1",
volume = "98",
doi = "10.1016/j.microc.2010.11.008"
}

Jarić, I., Višnjić Jeftić, Ž., Cvijanović, G., Gačić, Z., Jovanović, L., Skorić, S.,& Lenhardt, M.. (2011). Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of sterlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES. in Microchemical Journal
Elsevier Science Bv, Amsterdam., 98(1), 77-81.
https://doi.org/10.1016/j.microc.2010.11.008

Jarić I, Višnjić Jeftić Ž, Cvijanović G, Gačić Z, Jovanović L, Skorić S, Lenhardt M. Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of sterlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES. in Microchemical Journal. 2011;98(1):77-81.
doi:10.1016/j.microc.2010.11.008 .

Jarić, Ivan, Višnjić Jeftić, Željka, Cvijanović, Gorčin, Gačić, Zoran, Jovanović, Ljubinko, Skorić, Stefan, Lenhardt, Mirjana, "Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of sterlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES" in Microchemical Journal, 98, no. 1 (2011):77-81,
https://doi.org/10.1016/j.microc.2010.11.008 . .

TY  - JOUR
AU  - Morina, Filis
AU  - Jovanović, Ljubinko
AU  - Mojović, Miloš
AU  - Vidović, Marija
AU  - Panković, Dejana M
AU  - Veljović-Jovanović, Sonja
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/379
AB  - Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall
EP  - 224
IS  - 3
SP  - 209
VL  - 140
DO  - 10.1111/j.1399-3054.2010.01399.x
ER  - 

@article{
author = "Morina, Filis and Jovanović, Ljubinko and Mojović, Miloš and Vidović, Marija and Panković, Dejana M and Veljović-Jovanović, Sonja",
year = "2010",
abstract = "Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall",
pages = "224-209",
number = "3",
volume = "140",
doi = "10.1111/j.1399-3054.2010.01399.x"
}

Morina, F., Jovanović, L., Mojović, M., Vidović, M., Panković, D. M.,& Veljović-Jovanović, S.. (2010). Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum
Wiley, Hoboken., 140(3), 209-224.
https://doi.org/10.1111/j.1399-3054.2010.01399.x

Morina F, Jovanović L, Mojović M, Vidović M, Panković DM, Veljović-Jovanović S. Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum. 2010;140(3):209-224.
doi:10.1111/j.1399-3054.2010.01399.x .

Morina, Filis, Jovanović, Ljubinko, Mojović, Miloš, Vidović, Marija, Panković, Dejana M, Veljović-Jovanović, Sonja, "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall" in Physiologia Plantarum, 140, no. 3 (2010):209-224,
https://doi.org/10.1111/j.1399-3054.2010.01399.x . .

TY  - JOUR
AU  - Višnjić Jeftić, Željka
AU  - Jarić, Ivan
AU  - Jovanović, Ljubinko
AU  - Skorić, Stefan
AU  - Smederevac-Lalić, Marija
AU  - Nikčević, Miroslav
AU  - Lenhardt, Mirjana
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/401
AB  - Pontic shad (Alosa immaculata Bennet 1835) is an anadromous species that lives in the heavily polluted north-western part of the Black Sea and migrates into the Danube River to spawn To assess their heavy metal contamination levels, samples of Politic shad were collected at 863 river kilometre of the Danube River Muscle, liver and gill samples were prepared using microwave digestion. and the analysis of Al, As, Cd, Cu, B. Ba. Fe, Mg. Sr. Zn. Li, Co, Cr. Mn. Mo, Ni and Pb was performed with inductively-coupled plasma-optic emission spectroscopy (ICP-OES). Significant differences in concentrations of analyzed elements were observed among different tissues, as well as between the genders Al, Sr, Ba. Mg, and Li had the highest concentrations in gills, while Cd. Cu, Zn. Fe and B were highest in the liver While the muscle had the lowest concentrations of most of the analyzed elements, it had the highest concentration of As. This study has revealed that the concentrations of Cd and As in Politic shad muscle tissue were above the maximum acceptable concentrations for human consumption, thus precautions need to be taken in order to prevent future heavy metal contamination
PB  - Elsevier Science Bv, Amsterdam
T2  - Microchemical Journal
T1  - Heavy metal and trace element accumulation in muscle, liver and gills of the Pontic shad (Alosa immaculata Bennet 1835) from the Danube River (Serbia)
EP  - 344
IS  - 2
SP  - 341
VL  - 95
DO  - 10.1016/j.microc.2010.02.004
ER  - 

@article{
author = "Višnjić Jeftić, Željka and Jarić, Ivan and Jovanović, Ljubinko and Skorić, Stefan and Smederevac-Lalić, Marija and Nikčević, Miroslav and Lenhardt, Mirjana",
year = "2010",
abstract = "Pontic shad (Alosa immaculata Bennet 1835) is an anadromous species that lives in the heavily polluted north-western part of the Black Sea and migrates into the Danube River to spawn To assess their heavy metal contamination levels, samples of Politic shad were collected at 863 river kilometre of the Danube River Muscle, liver and gill samples were prepared using microwave digestion. and the analysis of Al, As, Cd, Cu, B. Ba. Fe, Mg. Sr. Zn. Li, Co, Cr. Mn. Mo, Ni and Pb was performed with inductively-coupled plasma-optic emission spectroscopy (ICP-OES). Significant differences in concentrations of analyzed elements were observed among different tissues, as well as between the genders Al, Sr, Ba. Mg, and Li had the highest concentrations in gills, while Cd. Cu, Zn. Fe and B were highest in the liver While the muscle had the lowest concentrations of most of the analyzed elements, it had the highest concentration of As. This study has revealed that the concentrations of Cd and As in Politic shad muscle tissue were above the maximum acceptable concentrations for human consumption, thus precautions need to be taken in order to prevent future heavy metal contamination",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Microchemical Journal",
title = "Heavy metal and trace element accumulation in muscle, liver and gills of the Pontic shad (Alosa immaculata Bennet 1835) from the Danube River (Serbia)",
pages = "344-341",
number = "2",
volume = "95",
doi = "10.1016/j.microc.2010.02.004"
}

Višnjić Jeftić, Ž., Jarić, I., Jovanović, L., Skorić, S., Smederevac-Lalić, M., Nikčević, M.,& Lenhardt, M.. (2010). Heavy metal and trace element accumulation in muscle, liver and gills of the Pontic shad (Alosa immaculata Bennet 1835) from the Danube River (Serbia). in Microchemical Journal
Elsevier Science Bv, Amsterdam., 95(2), 341-344.
https://doi.org/10.1016/j.microc.2010.02.004

Višnjić Jeftić Ž, Jarić I, Jovanović L, Skorić S, Smederevac-Lalić M, Nikčević M, Lenhardt M. Heavy metal and trace element accumulation in muscle, liver and gills of the Pontic shad (Alosa immaculata Bennet 1835) from the Danube River (Serbia). in Microchemical Journal. 2010;95(2):341-344.
doi:10.1016/j.microc.2010.02.004 .

Višnjić Jeftić, Željka, Jarić, Ivan, Jovanović, Ljubinko, Skorić, Stefan, Smederevac-Lalić, Marija, Nikčević, Miroslav, Lenhardt, Mirjana, "Heavy metal and trace element accumulation in muscle, liver and gills of the Pontic shad (Alosa immaculata Bennet 1835) from the Danube River (Serbia)" in Microchemical Journal, 95, no. 2 (2010):341-344,
https://doi.org/10.1016/j.microc.2010.02.004 . .