TY  - JOUR
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Bajuk-Bogdanovic, Danica
AU  - Branković, Goran
AU  - Rodić, Ivanka
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106
AB  - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
PB  - Springer-Verlag GmbH Berlin Heidelberg
T2  - Analytical and Bianalytical Chemistry
T1  - Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus
EP  - 6222.
IS  - 20
SP  - 6213
VL  - 414
DO  - 10.1007/s00216-022-04191-4
ER  - 

@article{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy",
publisher = "Springer-Verlag GmbH Berlin Heidelberg",
journal = "Analytical and Bianalytical Chemistry",
title = "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus",
pages = "6222.-6213",
number = "20",
volume = "414",
doi = "10.1007/s00216-022-04191-4"
}

Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry
Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222..
https://doi.org/10.1007/s00216-022-04191-4

Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222..
doi:10.1007/s00216-022-04191-4 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222.,
https://doi.org/10.1007/s00216-022-04191-4 . .